Quinn and Hynes squared off on WVON-AM's Cliff Kelley show, which garners a huge African-American audience, especially during the afternoon drive.
Comptroller Hynes was the first to arrive the South Side studios, passing about a dozen supporters outside.
"Hey! Hey! Ho! Ho! Quinn's got to go," they chanted.
Inside, the climate was as icy as the outdoor air.
In the video, Washington talks about why he removed Quinn as the city's revenue director more than 20 years ago.
"The powerful nature of Mayor Washington's words are very clear. It is about the fact that Pat Quinn was fired from his job," Hynes said.
Quinn urged listeners to not pay attention to the "false, negative" attack.
"Please don't listen to this negative, devisive attack on me. Instead, count on what I've done," the governor said.
Quinn has blasted Hynes for the ad because Quinn supported Washington while Hynes' father, Tom Hynes, broke away from the Democratic party to try to unseat Washington.
The polls are tight, but Hynes says that while the incumbent may have some big names in his corner, the race is a fight to the finish.