Daley said Thursday that even though the store would generate tax revenue and create jobs for neighborhood residents, there aren't enough votes in the City Council to pass the required redevelopment agreement.
Alderman Howard Brookins has tried for years to locate a Wal-Mart store in an industrial site being redeveloped into a shopping center. Currently, Wal-Mart operates a store on Chicago's West Side.
Opposition to Wal-Mart has come from labor unions, who claim the retailer does not pay workers adequately and skimp on benefits.
Daley's floor leader in the City Council, Alderman Pat O'Connor, says Brookins' latest effort comes at a time the city is trying to keep peace with the unions.